Our team of experts has selected the best tankless water heaters out of hundreds of models. Don't buy a water heater before reading these reviews. Stiebel Eltron TEMPRA-24-PLUS - Stiebel Eltron Tempra 24 Plus Electric Tankless Water Heater - Overview The Tempra® series has been redesigned completely. Shop our selection of Water Heaters and Plumbing in the Building Materials Department at the Home Depot Canada. Looking for the best electric tankless water heaters on the market today? Here is what to know about them and the leading brands on the market. Stiebel Eltron Tempra 2. Plus Tankless Water Heater. Stiebel Eltron Tempra 2. Plus Electric Tankless Water Heater. Stiebel Eltron Tempra 2. Plus. The Stiebel Eltron Tempra 2. Plus electric tankless water heater is one of the most powerful electric tankless water heaters available in North America. This 2. 8. 8. KW tankless heater is designed for whole house and commercial applications in cold climates where the incoming water temperature can drop below 4. F. It is also a popular choice for homes in warmer climates with high flow demands where multiple simultaneous hot water demands are required (up to 6 gallons per minute). Maximum Flow Rate in Gallons Per. Minute at 1. 05. F Output Temperature Incoming Water Temp: Flow Rate (GPM) 4. F3. 0 GPM4. 5F3. 3 GPM5. F3. 6 GPM5. 5F3. 9 GPM6. ![]() F4. 4 GPM6. 5F4. 9 GPM7. F5. 6 GPM7. 5F6. 5 GPM* This chart is based on 2. Please see brochure for temp rise chart based on 2. Like all Tempra Plus Series tankless water heaters, the Tempra 2. Plus features a digital temperature display and convenient dial selector which allows you to set the desired output water temperature at any level between 8. F (3. 0 and 6. 0C). Most importantly, it is one of the only tankless water heaters on the market that features a true thermostatic temperature control and full power- modulation based on both the incoming water temperature and the water flow rate. In layman's terms, the Tempra 2. The Tempra 2. 9 Plus also features dynamic Advanced Flow Control technology exclusive to Stiebel Eltron. If the heaters capacity is ever exceeded, this technology automatically adjusts the flow of water to maintain the desired temperature. This feature is particularly useful when a smaller unit than normal must be installed due to inadequate electrical service, or in cold climates. It is also desirable where high output temperatures are required for temperature critical applications (commercial dishwashers, etc.) and where flow rates are not always predictable. The result is the most precise temperature control of any tankless water heater on the market today! Thermostatically Controlled? Yes, digital temperature control and full power modulation based on flow rate and incoming water temperature. Advanced Flow Control Technology? Yes, this unit will automatically regulate the flow rate if it's capacity is exceeded. This feature is exclusive to Stiebel Eltron Tempra Plus Series tankless water heaters! KW 2. 8. 8 KW @ 2. KW @ 2. 08v. Voltage: 2. Hz. Max. Amps: 1. AMPS @ 2. 40v / 1. AMPS @ 2. 08v Breakers Required: * 3 x 4. AMP breakers (depends on local codes)Wiring: * Minimum 3 x #8 AWG Copper (depends on local codes)Dimensions: 1. Weight: 1. 7. 6 lbs (8. KG)Pipe Fittings: 3/4" NPTEnergy Efficiency: 9. Activation Flow Rate: 0. GPM / 2. 9 LPMMax. Water Pressure: 1. Max. Output Temperature: 1. F (6. 0C)Max. Input Temperature: 1. F (5. 5C)Warranty: Manufacturer's 3 Year Warranty. Safety: ETL Listed (USA & Canada) - complies with both US and Canadian safety standards including ANSI/UL4. CSA- E3. 35- 1/3. E- 9. 4. Protection: microprocessor- based thermostatic temperature control (flow rate and temperature modulating) with thermal hi- limit cut- out switch monitoring each heating module. Please note: A minimum 2. AMP household electrical service is highly recommended and will likely be required to install this model. If you are unsure of your electrical capacity in your home, please consult your electrician or contact us for assistance before ordering. Breaker size and wire gauge must meet all applicable local, state, provincial, and national electrical codes for your area - these are only guidelines that will apply to most installations. Some codes require use of electrical sub- panel for installation, especially when heater is not mounted within line- of- sight of the main electrical panel. Wiring should be sized to maintain a voltage drop of less than 3% under load. Please consult your electrician for more details.
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Never Stop Flirting With Your Partner. My wife is a great flirt. With me, with her friends, with an audience. She knows that flirtation isn’t just for people you’re just getting to know. Flirtation is just as important years into a relationship as it is at the start. Fatherly’s refresher course on flirting with your partner doesn’t have any big surprises; it’s just a good reminder to get back in the habit. Just try a few compliments, some playful texts, or touching your partner as if you haven’t seen them naked a thousand times in bed, in the kitchen, and on the toilet. メモ帖 間違いの指摘は へ。※スパム排除の為 @yahoo.com,@hotmail.com は受信拒否しています。 ↑きゃっとま.The rules are basically the same as flirting with someone new, except that you already know your partner’s preferences and boundaries. You don’t have to resort to stunts like answering the door wrapped in cellophane. In a long- term relationship, it’s an incredible turn- on just to treat your partner as if you were still discovering them. Which, of course, you are. You can go far just by gazing at them like you did on your first date. And of course you can reminisce about that first date, and any other memories of when you fell in love, which will excite some of the same feelings you had then. Just don’t flaunt your flirtation in public too much. No one likes a couple who make out at a party, or call each other good flirts in a blog post. ![]() In September, Lyft has partnered with local restaurants in 12 major cities to offer you a free or discounted food for just showing your Lyft app. Tabtight professional, free when you need it, VPN service. Dateierweiterungen. Eine der umfangreichsten Listen mit Dateierweiterungen.. ABCDEFGHIJKLMNOPQRSTUVWXYZErweiterung. Was. 00. 0(0. 00- 6. Paperport Scanned Image. ARJ Multi- volume Compressed Archive. Sequentially Numbered Backup Files. Trend Micro Pattern File. 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Threat- removal layer - Targets and eliminates hard- to- remove threats. Norton Safe Web for Facebook - Scans your Facebook News Feed for dangerous links and unsafe posts. Norton Identity Safe - Remembers, secures and automatically enters your user names and passwords for you. ![]() ![]() Reprinted from www. A trademark of Ziff Davis, Inc. Used under license. Pc Tools Spyware Doctor DisconnectedExchange Rate. Money Management | www. In finance, an exchange rate (also known as a foreign- exchange rate, forex rate, ER, FX rate or Agio) between two currencies is the rate at which one currency will be exchanged for another. It is also regarded as the value of one country’s currency in relation to another currency.[1] For example, an interbank exchange rate of 1. Japanese yen (JPY, ?) to the United States dollar (US$) means that ? US$1 or that US$1 will be exchanged for each ? In this case it is said that the price of a dollar in relation to yen is ? Exchange rates are determined in the foreign exchange market,[2] which is open to a wide range of different types of buyers and sellers, and where currency trading is continuous: 2. GMT on Sunday until 2. GMT Friday. The spot exchange rate refers to the current exchange rate. The forward exchange rate refers to an exchange rate that is quoted and traded today but for delivery and payment on a specific future date. In some areas of Europe and in the retail market in the United Kingdom, EUR and GBP are reversed so that GBP is quoted as the fixed currency to the euro. In order to determine which is the fixed currency when neither currency is on the above list (i. This reduces rounding issues and the need to use excessive numbers of decimal places. There are some exceptions to this rule: for example, the Japanese often quote their currency as the base to other currencies. The real exchange rate (RER) is the purchasing power of a currency relative to another at current exchange rates and prices. It is the ratio of the number of units of a given country's currency necessary to buy a market basket of goods in the other country, after acquiring the other country's currency in the foreign exchange market, to the number of units of the given country's currency that would be necessary to buy that market basket directly in the given country. There are various ways to measure RER.[1. Thus the real exchange rate is the exchange rate times the relative prices of a market basket of goods in the two countries. For example, the purchasing power of the US dollar relative to that of the euro is the dollar price of a euro (dollars per euro) times the euro price of one unit of the market basket (euros/goods unit) divided by the dollar price of the market basket (dollars per goods unit), and hence is dimensionless. This is the exchange rate (expressed as dollars per euro) times the relative price of the two currencies in terms of their ability to purchase units of the market basket (euros per goods unit divided by dollars per goods unit). If all goods were freely tradable, and foreign and domestic residents purchased identical baskets of goods, purchasing power parity (PPP) would hold for the exchange rate and GDP deflators (price levels) of the two countries, and the real exchange rate would always equal 1. This report provides exchange rate information under Section 6. Public Law 8. 7- 1. September 4, 1. 96. USC 2. 36. 3 (b)) which gives the Secretary of the Treasury sole authority to establish the exchange rates for all foreign currencies or credits reported by all agencies of the government. The primary purpose is to ensure that foreign currency reports prepared by agencies are consistent with regularly published Treasury foreign currency reports regarding amounts stated in foreign currency units and U. S. dollar equivalents. The rates provided in this report are not meant to be used by the general public for conducting foreign currency conversion transactions. This paper deals with application of quantitative soft computing prediction models into financial area as reliable and accurate prediction models can be very helpful in management decision- making process. The authors suggest a new hybrid neural network which is a combination of the standard RBF neural network, a genetic algorithm, and a moving average. The moving average is supposed to enhance the outputs of the network using the error part of the original neural network. Authors test the suggested model on high- frequency time series data of USD/CAD and examine the ability to forecast exchange rate values for the horizon of one day. ![]() To determine the forecasting efficiency, they perform a comparative statistical out- of- sample analysis of the tested model with autoregressive models and the standard neural network. They also incorporate genetic algorithm as an optimizing technique for adapting parameters of ANN which is then compared with standard backpropagation and backpropagation combined with K- means clustering algorithm. Finally, the authors find out that their suggested hybrid neural network is able to produce more accurate forecasts than the standard models and can be helpful in eliminating the risk of making the bad decision in decision- making process. Forex contracts involve the right to buy or sell a certain amount of a foreign currency at a fixed price in U. S. dollars. Profits or losses accrue as the exchange rate of that currency fluctuates on the open market. It is extremely rare that individual traders actually see the foreign currency. Instead, they typically close out their buy or sell commitments and calculate net gains or losses based on price changes in that currency relative to the dollar over time. Forex markets are among the most active markets in the world in terms of dollar volume. The participants include large banks, multinational corporations, governments, and speculators. Individual traders comprise a very small part of this market. Because of the volatility in the price of foreign currency, losses can accrue very rapidly, wiping out an investor’s down payment in short order. Any company operating globally must deal in foreign currencies. Statistical Techniques | Statistical Mechanics. It has to pay suppliers in other countries with a currency different from its home country’s currency. The home country is where a company is headquartered. The firm is likely to be paid or have profits in a different currency and will want to exchange it for its home currency. Even if a company expects to be paid in its own currency, it must assess the risk that the buyer may not be able to pay the full amount due to currency fluctuations. XE Currency Converter - Live Rates. Calculate live currency and foreign exchange rates with this free currency converter. You can convert currencies and precious metals with this currency calculator. Eur/Usd · USD - Us Dollar · GBP - British Pound · Cad/Usd · Currency Charts. Currency Converter | Foreign Exchange Rates | OANDA. Free currency converter or travel reference card using daily OANDA Rate® data. Convert currencies using interbank, ATM, credit card, and kiosk cash rates. Historical Exchange Rates · Average Exchange Rates · Live Exchange Rates. Exchange Rates - X- Rates. Free foreign exchange rates and tools including a currency conversion calculator, historical rates and graphs, and a monthly exchange rate average. Currency Calculator · US Dollar · Eur/Usd · Rates Table · Historic Lookup.
Exchange Rate – Live Foreign Exchange Rates | OFX. Click to find current live exchange rates for supported foreign currencies on OFX. Forex Currency Exchange Rates - Forex- Ratings. Forex Currencies quotes change many times a day. Exchange rate is the cost of the chosen currency at a certain moment of time in comparison with the other currency. ![]() 1g Dsm Ss Clutch Line Install Pc RemoteCurrency Exchange Rate Calculator - ofx. Free currency converter tool to quickly check foreign exchange rates. Click to use the currency converter. Live Exchange Rates | OANDA. Get live exchange rates for major currency pairs from the OANDA fx. Trade platform. Updated every 5 seconds. Foreign Currency and Currency Exchange Rates. Foreign Currency and Currency Exchange Rates. You must express the amounts you report on your U. S. tax return in U. S. dollars. If you receive all or part .. Daily Foreign Exchange Rates. English/Statistics/Financial. Markets/Exchange. Rate/.. NOTICE: Myanmar's central bank has set a reference exchange rate under a managed float currency regime starting from 0. April 2. 01. 2. FOREX. Official Site. https: //www. FOREX. com offers forex & metals trading with award winning trading platforms, tight spreads, quality executions, powerful trading tools & 2. Foreign Exchange Rates and Currency Exchange Rate .. View foreign exchange rates and use our currency exchange rate calculator for more than 3. Yearly Average Currency Exchange Rates - irs. Yearly Average Currency Exchange Rates Translating foreign currency into U. 1g Dsm Ss Clutch Line Install Pc Camera[Author’s note: This article is the first of a series on the Doctrine of the Holy Spirit. Embraced in this opening article are the following major divisions of the.![]() ![]() Very good, detailed article on the Baptism of the Holy Spirit and how to properly receive it from the Lord. The Teacher’s Notes–The Baptism and Filling of the Holy Spirit, Lesson 5 Adult Sabbath School Bible Study Guide, 2017 1Q, "The Holy Spirit and Spirituality". What The Bible Says On The Baptism Holy Spirit. Table of Contents. In this article, I will show you exactly what this gift of Baptism of the Holy Spirit is and how to properly receive it. Many have tried to receive this gift at an altar call at their local churches, but they have failed to receive it. They then walk away thinking that maybe there was something wrong with them or they were not worthy enough to receive this gift from the Lord. I believe the number one reason why some Christians do not properly receive this gift when they try the above approach is because they have not been properly prepared to receive it. To really receive this gift the first time you try asking and pressing in for it, you have to know exactly what it is you are receiving, and then you have to properly prepare yourself to receive it. Once those two things have been properly done, then it becomes very easy to receive this gift the first time you ask and press in for it. This is a gift that God wants all of His children to have, just like His gift of eternal salvation through His Son Jesus Christ. I will first start off by explaining exactly what this gift is, and then I will show you how to properly prepare yourself to receive it. Once those two things have been done, I will then show you exactly how to receive it once you are ready for it. The first thing that you will need to know is exactly what this gift is. The Bible tells us that we will all receive the Holy Spirit at the moment of our salvation with the Lord. The Bible also tells us that we are “sealed” with the Holy Spirit the minute we accept Jesus Christ as our personal Lord and Savior. So if we have already received the Holy Spirit at the moment of our salvation with the Lord, then what is this “second” baptism of the Holy Spirit that the charismatics are talking about? The Bible tells us that there is only one baptism. To fully understand what is meant by the baptism of the Holy Spirit – you have to know what the difference is between your body, soul, and spirit. The Bible tells us that we have three distinct parts to our being – body, soul, and spirit. Here is the verse specifically telling us this: “Now may the God of peace Himself sanctify you completely, and may your whole spirit, soul, and body be preserved blameless at the coming of our Lord Jesus Christ.” (1 Thessalonians 5: 2. Your body is your natural, physical, flesh body. Your soul is located on the inside of your body. Your soul is who you really are. It is your total personality – it is your mind, will, emotions, and intellect. Your soul is spirit in form, which means it cannot be seen by any natural means. It is completely invisible to the naked eye. On the inside of your soul is your spirit. The Bible refers to our human spirit as being our “innermost being.” I believe another word for our human spirit is “heart.” When people refer to having had a broken heart in a romantic relationship that has been dissolved, a broken heart is really a “wounded spirit.”I also believe Jesus gives us a clue as to where our human spirits may be located at in our beings in the following verse. The New King James Version uses the word “heart”, but the Original King James Version uses the word “belly.”“He who believes in Me, as the Scripture has said, out of his heart (BELLY) will flow rivers of living water. But this He spoke concerning the Spirit, whom those believing in Him would receive; for the Holy Spirit was not yet given, because Jesus was not yet glorified.” (John 7: 3. When we receive Jesus Christ as our personal Lord and Savior, the Holy Spirit comes and enters into our human spirits. Our spirits are thus baptized with His actual presence at the exact moment of our salvation with the Lord. This is the baptism that the Bible is talking about when it says that there is only one baptism. However, what the charismatics are referring to is a second experience where the Holy Spirit now comes up and enters into your soul. Your spirit has already been baptized with the Holy Spirit at the moment of your salvation with the Lord, but now God wants to take it one step further. He now wants to baptize your soul with his Holy Spirit. Another word that can be used for the “baptism” of the Holy Spirit where He will now enter your soul area is the “release” of the Holy Spirit into your soul. The Holy Spirit will release from your spirit to come up and enter into your soul. I believe this is what Paul was referring to when he commands us to be “filled with the Spirit.” If our spirits are already filled up with the Holy Spirit at the moment of our salvation with the Lord, then what else needs to be filled up? It is our souls. When you have received the second gift of the Baptism of the Holy Spirit, you are now considered “Spirit- filled.” Both your spirit and your soul are now filled with the presence of the Holy Spirit. When Jesus tells us in the above verse that out of our “belly” will flow rivers of living water, I believe that He may be telling us that our human spirits are located in our belly areas. For those of you who have ever felt any kind of heart pain in a broken love relationship – if you will recall, the pain you were feeling was in your belly area. It feels like there is a knife on the inside of your belly area just tearing you up. Again, a broken heart is really a wounded spirit, and when your heart or spirit has been wounded and hurt, you will literally feel physical pain in your belly area. The House Analogy. Another way to try to understand all of the above is what I call the house analogy. When you buy a house, you will get two things – the house, and the front and back yards. The analogy is that your house is your soul, and your front yard is your spirit. When you receive God’s free gift of salvation through accepting Jesus Christ as your personal Lord and Savior, the Holy Spirit comes and enters into your front and back yards, which is your human spirit. However, He now wants to go one step further and come into your actual house, which is your soul area. Why? Because this is where you really live at. You spend most of your time in the house as versus being outside in the front and back yards. Jesus says in the following verse: “Behold, I stand at the door and knock. If anyone hears My voice and opens the door, I will come into him and dine with him, and he with Me.” (Revelation 3: 2. I believe Jesus is not only telling us that He is literally going to come into our human spirits at the moment of our salvation with Him, but that He can also enter into our souls through the Holy Spirit. Even though Jesus is in heaven, He now lives on the inside of us “through” the Holy Spirit. Just as Jesus is living on the inside of us and making contact with us through the Holy Spirit, we likewise can make direct contact with both Him and God the Father through the Holy Spirit. It is “by, in, and through” the Holy Spirit that we have direct access to both God and Jesus who live and dwell in heaven. Here are several good Scripture verses proving the above points: a) This first verse tells us that the Holy Spirit literally lives on the inside of each of us.“Do you not know that you are the temple of God and that the Spirit of God dwells in you?” (1 Corinthians 3: 1. Now that we know that the Holy Spirit lives on the inside of each of us, and that both God and Jesus live in heaven – watch how we make direct contact with both God and Jesus in heaven, which is through the Holy Spirit: “For through Him we both have access by one Spirit to the Father.” (Ephesians 2: 1. And by this we know that He abides in us, by the Spirit whom He has given us.” (1 John 3: 2. These two verses specifically tell us that we can make direct contact with both God and Jesus who live in heaven “by the Spirit.” The second verse tells us that Jesus literally lives and abides in us “by the Spirit.”2. The Telephone Cord Analogy. Another analogy to fully understand how we can make direct contact with God and Jesus in heaven through the Holy Spirit is what I call the telephone cord analogy. We can pick up a telephone and call someone 8. Even though that person is physically far away from us, we can still hear their voice as if they were only two inches from our ears. It’s as if that person is literally in our actual presence. It is the same way with God, Jesus, and the Holy Spirit. Even though God and Jesus dwell in heaven and do not live on this earth, we can still make direct contact with the both of Them through the Holy Spirit. Polish Witcher Documentary Shows Journey From Books To Game. Geralt’s trip from the pages of Polish writer Andrzej Sapkowski’s novels to the games we know today was not an easy one. Polish gaming site Arhn. You. Tube as “The Witcher’s video game history,” telling the story of the first Witcher game’s development. Update 4: 5. 0 ET: a commenter has translated the thumbnail to “White Wolf: The history of digital Witcher.)Although it’s in Polish, English subtitles are available. In it, the developers of The Witcher tell stories of hardship and trials, and even discussions with Sapkowski over the writing and characterization of leading man Geralt of Rivia. One developer relates a correspondence with Sapkowski over the end of Chapter 3, in the Kikimore lair, where the player can find a letter written to the Professor. The team inserted a phrase written by Sapkowski basically verbatim, a proud moment for them. However, I must insist you cease referring to him as a ‘son- of- a- bitch’—appellations of that nature are entirely inaccurate, inappropriate and painfully primitive.”Other little tidbits include a mysterious missing mission and other strange facts about the development of the game. At almost two hours it’s a hefty run- time, but worth your time if you want to see how The Witcher made the jump to games and laid the foundation for some excellent games to come. Alignment (Dungeons & Dragons) - Wikipedia. In the Dungeons & Dragons (D& D) fantasyrole- playing game, alignment is a categorization of the ethical and moral perspective of player characters, non- player characters, and creatures. Most versions of the game feature a system in which players make two choices for characters. One is the character's views on "law" vs "chaos", the other on "good" vs "evil". The two axes allow for nine alignments in combination.[1][2] These nine alignments can be shown in a grid, as follows: History[edit]D& D co- creator Gary Gygax credited the inspiration for the alignment system to the fantasy stories of Michael Moorcock[3] and Poul Anderson.[4]The original version of D& D allowed players to choose among three alignments when creating a character: lawful, implying honor and respect for society's rules; chaotic, implying rebelliousness and individualism; and neutral, seeking a balance between the extremes.[citation needed]The 1. Dungeons & Dragons Basic Set introduced a second axis of good, implying altruism and respect for life, vs evil, implying selfishness and no respect for life. As with the law- vs- chaos axis, a neutral position exists between the extremes. @Patricia Berryman. Depends on the edition, of course. For 3rd (or 3.5) edition, the free “mini adventures” from Wizards of the Coast website are a great place to. ![]() Characters and creatures could be lawful and evil at the same time (such as a tyrant), or chaotic but good (such as Robin Hood).[5]Advanced Dungeons & Dragons (AD& D), released between 1. The 1. 98. 1 version of the Basic Set, however, went back to the earlier one- axis alignment system.[7]AD& D 2nd Edition, released in 1. In that edition, a character who performs too many actions outside their alignment can find their alignment changed, and is penalized by losing experience points, making it harder to reach the next level.[8]D& D 3rd Edition, released in 2. D& D 4th Edition, released in 2. In that edition, "good" replaces neutral good and does not encompass chaotic good; "evil" replaces neutral evil and does not encompass lawful evil; "unaligned" replaces true neutral and does not encompass lawful neutral and chaotic neutral.[1. D& D 5th Edition, released in 2. Function[edit]Richard Bartle's Designing Virtual Worlds noted that alignment is a way to categorize players' characters, along with gender, race, character class, and sometimes nationality. Alignment was designed to help define role- playing, a character's alignment being seen as their outlook on life. A player decides how a character should behave in assigning an alignment, and should then play the character in accordance with that alignment.[1. A character's alignment can change. Dungeons & Dragons is an American animated television series based on TSR's Dungeons & Dragons role-playing game. A co-production of Marvel Productions and TSR, the. Geralt’s trip from the pages of Polish writer Andrzej Sapkowski’s novels to the games we know today was not an easy one. The Schenectady Wargamers Association, Inc. (SWA) is an Adventure Gaming club serving the Capital District area (Albany, Schenectady, Troy, Saratoga) of upstate New. If a lawful neutral character consistently performs good acts, when neutral or evil actions were possible, the character's alignment will shift to lawful good. In games, the Dungeon Master (referee) decides when alignment violations occur, as it is subjective.[1. Characters acting as a party should have compatible alignments. Lawful good characters are compatible with lawful evil characters if they have a common goal, but the addition of a chaotic evil character may tear the party apart. The authors of Dungeon Master For Dummies have found that a party of good or neutral characters works better: the impetus for adventures is easier, group dynamics are smoother, and it allows the "heroic aspects of D& D [to] shine through".[1. Law vs. chaos[edit]The law versus chaos axis in D& D predates good versus evil in the game rules. Originally the law/chaos axis was defined as the distinction between "the belief that everything should follow an order, and that obeying rules is the natural way of life", as opposed to "the belief that life is random, and that chance and luck rule the world".[7] According to the early rulebook, lawful characters are driven to protect the interest of the group above the interest of the individual and would strive to be honest and to obey just and fair laws. Chaotic creatures and individuals embraced the individual above the group and viewed laws and honesty as unimportant. At that time, the rulebook specified that "chaotic behavior is usually the same as behavior that could be called 'evil'".[7] Neutral creatures and characters believe in the importance of both groups and individuals, and felt that law and chaos are both important. They believe in maintaining the balance between law and chaos and were motivated by self- interest.[7]The third edition D& D rules define "law" and "chaos" as follows: [9]Law implies honor, trustworthiness, obedience to authority, and reliability. On the downside, lawfulness can include closed- mindedness, reactionary adherence to tradition, judgmentalness, and a lack of adaptability. Those who consciously promote lawfulness say that only lawful behavior creates a society in which people can depend on each other and make the right decisions in full confidence that others will act as they should. Chaos implies freedom, adaptability, and flexibility. On the downside, chaos can include recklessness, resentment toward legitimate authority, arbitrary actions, and irresponsibility. Those who promote chaotic behavior say that only unfettered personal freedom allows people to express themselves fully and lets society benefit from the potential that its individuals have within them. Someone who is neutral with respect to law and chaos has a normal respect for authority and feels neither a compulsion to follow rules nor a compulsion to rebel. They are honest but can be tempted into lying or deceiving others if it suits him/her. Good vs. evil[edit]The conflict of good versus evil is a common motif in D& D and other fantasy fiction. Although player characters can adventure for personal gain rather than from altruistic motives, it is generally assumed that the player characters will be opposed to evil and will tend to fight evil creatures. The third edition D& D rules define "good" and "evil" as follows: [9]Good implies altruism, respect for life, and a concern for the dignity of sentient beings. Good characters make personal sacrifices to help others. Evil implies harming, oppressing, and killing others. Some evil creatures simply have no compassion for others and kill without qualms if doing so is convenient or if it can be set up. Others actively pursue evil, killing for sport or out of duty to some malevolent deity or master. People who are neutral with respect to good and evil have compunctions against killing the innocent but lack the commitment to make sacrifices to protect or help others. Neutral people are committed to others by personal relationships. Within the game, Paladins, altruistic heroes, and creatures such as angels are considered good. Villains and violent criminals are considered evil, as are inherently evil creatures such as demons and most undead.[9] Animals are considered neutral even when they attack innocents, because they act on natural instinct and lack the intelligence to make moral decisions; [9] In the fifth edition, this is expressed by labeling such beasts as "unaligned".[1. Alignments[edit]Lawful good[edit]A lawful good character typically acts with compassion and always with honor and a sense of duty. Such characters include righteous knights, paladins, and most dwarves. Lawful good creatures include the noble golden dragons.[1. Neutral good[edit]"Neutral Good" redirects here. For the term in economics, see Neutral good. A neutral good character typically acts altruistically, without regard for or against lawful precepts such as rules or tradition. A neutral good character has no problems with cooperating with lawful officials, but does not feel beholden to them. Final Cut Express 2 0 Manual Dexterity Jobs![]() Whole- body tissue stabilization and selective extractions via tissue- hydrogel hybrids for high- resolution intact circuit mapping and phenotyping. Because of their intrinsic transparency, the worm Caenorhabditis elegans and the zebrafish Danio rerio provide scientists with an unobstructed, organism- wide view of tissue anatomy and cellular activity via, e. In combination with their small size and genetic tractability, their whole- body transparency enables rigorous, high- throughput investigations into how environmental, cellular and genetic alterations influence biological processes from cellular signaling and apoptosis, to organism development and survival. By contrast, the comparatively large size and optical opacity of mammalian models generally has limited researchers to imaging snapshots of cellular organization on thin- sectioned tissue samples. However, it was hypothesized that if the bodies of these mammalian model organisms were to acquire the same level of optical transparency as zebrafish embryos, whole- body image data sets would theoretically become available to scientists for study (Table 1). Several methodologies for tissue clearing have been proposed for large- scale 3. D mapping of tissue macromolecular content. Final Cut Express 2 0 Manual Dexterity EvaluationEach of these protocols offers distinct advantages, such as preserving tissue architecture. Building on our prior CLARITY technique and concepts for generating extractable tissue- hydrogel hybrids. PACT, PARS and RIMS to offer a user- friendly, rapid approach to rendering whole organs and whole organisms transparent. These methods help to stabilize tissue architecture and preserve the macromolecular content of samples, thus enabling imaging of immunohistochemical, single- molecule RNA fluorescence in situ hybridization (sm. FISH), and small- molecule staining throughout thick tissues, as well as enabling long- term storage. In this protocol, we provide detailed information about how to implement PACT, PARS and RIMS so that users can apply these methods to their own research. ![]() The following is the Department´s 1991 title III ADA regulation published July 26, 1991, which should continue to be used until March 14, 2011. CSU offers a fully accredited Bachelor of Dental Science that qualifies you for registration as a dental practitioner in all Australian states and territories. Join Our Tribe; About Us; LIFE AT US FITNESS. _____. Imaging of large volumes of cleared tissue can generate gigabyteto terabyte- sized data sets, which creates new challenges associated with the computational analysis of the high- resolution image stacks. Tract- tracing in particular is a difficult and laborious undertaking, whether for mapping the brain connectome or for generating a smaller- scale wiring diagram of isolated projections between specific brain regions or of peripheral nerves at target organs. Thus, in conjunction with refining methodologies to render tissues transparent, we evaluated a wide range of image analysis software packages for their ability to process data sets of cleared brain volumes. On the basis of our findings regarding the best- performing tools, we propose here sample workflows to provide users with a springboard for basic image analysis to complement and facilitate their adoption of the PACT, PARS and RIMS methods. Advantages of tissue clearing by tissue- hydrogel hybrids. The tissue stabilization and clearing methods that we developed. PACT) or through the vasculature of intact postmortem organisms (PARS). The hydrogel mesh itself is transparent, and it secures proteins and nucleic acids into place so that we can later detect them with fluorescent labels under a microscope. There are a number of tissue clearing protocols available that combine the use of `chemical' clearing methods (i. RIs) throughout the sample and sample mount, a feat that is usually accomplished through sample hyperhydration, dehydration and/or immersion in specially designed mounting solutions) in order to maximize sample transparency. We experimented with many of these protocols alongside our initial development. PACT and PARS so that we could endeavor to incorporate some of their strengths and avoid major pitfalls. For example, we recognized the hydrogel- based tissue stabilization of CLARITY8,2. DISCO1. 4 and the decolorization of CUBIC1. Our observations are summarized in Table 2 to guide researchers in selecting a clearing protocol that best suits their clearing application. Clearing techniques that multi- task: macromolecular extraction and refractive index matching. Emerging from these different approaches to tissue clearing, PACT and PARS are notable for their versatility in preparing a variety of tissue types for high- resolution imaging at depth. The PACT hydrogel formulation and clearing process is easily optimized to render difficult- to- image tissues transparent (e. PACT- de. CAL, for PACT delipidation and decalcification of bone), to expand tissues for better separation of compact structures (e. PACT, for PACT- based expansion clearing of dense cells or projections) and to preserve tissue integrity in fragile samples through varying the degree of paraformaldehyde- tissue cross- linking. Meanwhile, PARS is positioned to tackle a variety of scientific problems that would benefit from a comprehensive, whole- body view of gene expression patterns, cellular organization and/or structural composition. PACT- or PARS- based preparation and clearing of tissue, followed by tissue mounting in RIMS, can preserve the signal from native fluorescent proteins and improve the efficacy of postclearing immunofluorescence labeling (Fig. Fluorescence signal intensity is also maintained through month- long storage periods post fixation. Other brain- specific tissue clearing protocols (Table 2) have at least one functional drawback, such as incompatibility with endogenous fluorescent labels. Some of these limitations have been overcome by the use of automated tissue sectioning techniques. These heavily automated imaging systems are not readily available to biology and clinical laboratories with limited resources and budgets. Conversely, traditional histology relies on the irreversible sectioning and manual reconstruction of successive slides for image analysis, which is time- consuming and potentially loses molecular information and connectivity in the process. PACT, PARS and RIMS enable deep imaging of large tissue samples without sectioning and reconstruction. Antibody expense aside, these cost- effective techniques generate detailed 3. D reconstructions of intact circuits using only mainstream single- photon microscopy. Experimental design. The procedure comprises seven main stages: tissue preparation (Steps 1–5); formation of a tissue- hydrogel matrix (Step 6); tissue clearing (Steps 7 and 8); staining (Step 9, optional); enhancement of optical clarity using RIMS (Steps 1. Step 1. 4); and image visualization and analysis (Steps 1. Although PACT and PARS, including their respective tissue- specific variations (PACT- de. CAL, PARS- CSF1. 8), each follow the same main stages, the decision to proceed with PACT or PARS is generally made before commencing the procedure. If the primary goal is to stabilize soft and/or amorphous samples (e. Steps 1–6 (PACT or PARS), and with the option to include bis- acrylamide and/or paraformaldehyde (PFA) in the PACT hydrogel formulation for increased sample rigidity. Required expertise Most steps of the procedure can be performed by all members of the research community. Aspects of the PARS setup (Steps 1–5) require that the scientist be approved for working with laboratory animals and/or possess the surgical dexterity to establish an intravascular route for delivery of PARS reagents. For example, to execute PARS- based clearing of whole laboratory animals (e. As whole- organ and thick- tissue imaging can generate tera- scale data sets, a computational or informatics background, although not necessary if relying on commercial software with good technical support, is very helpful in managing large data sets (file storage and handling) and in performing image analysis (Steps 1. PACT or PARS? Without the use of organic solvents, passively clearing and immunostaining larger tissue volumes (e. In terms of clearing efficiency, PACT1. Figs. 1f,g and 2–6; Supplementary Figs. Supplementary Fig. Fig. 1f,g)). For whole- organ screening or profiling tissues throughout the entire organism, PARS greatly accelerates and simplifies the clearing process. All hydrogel monomer solutions, wash buffers, buffered detergents and phenotypic labels are driven throughout tissue vasculature via a perfusion- based pressure gradient (Fig. PARS clearing renders most organs transparent within 4 d (Fig. Supplementary Fig. Isolation of Double Negative αβ T Cells from the Kidney. Abstract. There is currently no standard protocol for the isolation of DN T cells from the non- lymphoid tissues despite their increasingly reported involvement in various immune responses. DN T cells are a unique immune cell type that has been implicated in regulating immune and autoimmune responses and tolerance to allotransplants. DN T cells are, however, rare in peripheral blood and secondary lymphoid organs (spleen and lymph nodes), but are major residents of the normal kidney. Very little is known about their pathophysiologic function. We recently described a comprehensive phenotypic and functional analysis of this population in the kidney. Analysis of DN T cell function will be greatly enhanced by developing a protocol for their isolation from the kidney. Here, we describe a novel protocol that allows isolation of highly pure ab CD4+ CD8+ T cells and DN T cells from the murine kidney. Briefly, we digest kidney tissue using collagenase and isolate kidney mononuclear cells (KMNC) by density gradient. This is followed by two steps to enrich hematopoietic T cells from 3% to 7. KMNC. The first step consists of a positive selection of hematopoietic cells using a CD4. In the second step, DN T cells are negatively isolated by removal of non- desired cells using CD4, CD8, and MHC class II monoclonal antibodies and CD1d α- galcer tetramer. This strategy leads to a population of more than 9. DN T cells. Surface staining with the above mentioned antibodies followed by FACs analysis is used to confirm purity. Keywords: Immunology, Issue 8. Double Negative (DN) αβ, T cells, CD4. T cell isolation, renal lymphocytes, non- lymphoid- tissues, T cells purification, Ischemia Reperfusion Injury, Acute Kidney Injury, Tissue Resident Lymphocytes, Lymphoproliferative Disorders, Erythematosus Lupus. Introduction. Peripheral αβTCR+CD3+CD4- CD8- double- negative (DN) T cells are divided into various subsets that possess distinct phenotypes and functions. DN T cells are poorly understood but increasingly being implicated in pathophysiological immune responses in different disease models. DN T cells are a unique immune cell type that is increasingly implicated in the regulation of various immune and autoimmune responses and the modulation of allotransplant tolerance. They are rare in the peripheral blood and secondary lymphoid organs (spleen and lymph nodes). However, they are major residents of the normal kidney and gut epithelium. Very little is known about their function. AKI) associated with kidney transplant. Because of the intricate roles of different immune cells in regulating immune responses including alloresponses, defining the role of each player is critical in understanding alloresponses and designing new therapeutics. Given the significant numbers of DN T cells present in the kidney under physiologic and different disease conditions, DN T cells are likely to play a critical role in regulating immune and autoimmune responses in mice and humans, and alloresponses in transplant recipients. Accumulating though still scattered evidence implicates DN T cells in both pathogenic and immunosuppressive functions but it is poorly understood why and how they exhibit a specific harmful or suppressive function and how the environment influences them. Due to their low abundance in the kidney, improved methods of isolation are necessary. There is currently no standard protocol for isolation of DN T cells from the non- lymphoid tissues. Our protocol describes a novel method for isolation of DN T cells from the kidney; however, the method can also be used for various non- lymphoid tissues. Protocol. 1. Preparation of Instruments, Culture Media and Reagents. Instruments: Prepare the reagents under sterile conditions and use under a laminar flow hood. Prepare 1 L of RPMI tissue culture medium, add 5% of fetal bovine serum, 2. HEPES, 1 mg sodium pyruvate and 1. Note: the recommended tissue culture medium, RPMI, is interchangeable with DMEM. Prepare 5% collagenase “D” solution (5 ml of collagenase “D” to 9 ml of tissue culture medium). Make Percoll solution at three different concentrations: 1. The following concentrations are calculated for 1 sample. Keep solutions at room temperature. Percoll solution: add 9 ml of undiluted Percoll (from stock solution); add 1 ml of PBS 1. Percoll solution: add 8 ml of Percoll 1. PBS 1x. Percoll solution: add 4 ml of Percoll 8. PBS 1x. Make 1 L of fluorescence- activated cell sorting (FACS) buffer; add 5 ml 1. BSA (0. 1% BSA), 1 ml 1. EDTA agent, make up to 1,0. PBS. 2. Preparation of Kidney Digestion. Having acquired any necessary institutional approval, sacrifice and exsanguinate the mice with standard methods and following the current regulations and animal care guidelines. Anesthetize the mouse with pentobarbital (0. Proceed to exsanguination. Place the mouse in a supine position on a surgical table. Spray the abdominal area with 7. Add 1. 0 ml of collagenase D solution to the Petri dish. Remove and decapsulate both kidneys from each mouse. Open the abdomimal cavity by making a midline incision through the abdominal skin and peritoneum from the sternum to the pubis. Expose the left kidney by moving the intestine laterally to the side. Carefully separate the left capsule with the forceps. Note: The capsule appears as a transparent layer surrounding the kidney and partially covered by perinephric adipose tissue. Remove the left kidney by cutting the pedicle vessels using a surgical scissor. Repeat same procedure for the right kidney. Note: The right kidney is more cranial and closer to the midline (pedicels are shorter). Place the kidney into the Petri dish. Cut the kidney tissue into small pieces (1- 2 mm in dimension) using a regular metal shaping blade and place the pieces into the collagenase solution for 3. C (Figure 1). 3. Preparation of Kidney Mononuclear Cells (KMNC) from the Kidney Digestion. Obtain a cell suspension of the kidney digestion by mechanically disrupting the tissue using a strainer (7. Centrifuge at 4. 00 x g for 1. C to pellet the cell suspension. Re- suspend the pellet in 4 ml of 4. Percoll solution and gently overlay onto 4 ml of 8. Percoll solution with a transfer plastic pipette, very slowly and carefully. This will result in two phases clearly separated by a translucent layer. On the top will be a yellow layer corresponding to the lipids. Centrifuge at 1,5. Remove by aspiration the yellow and thick top layer (about 1- 2 ml). Collect only the slight whitish translucent layer from the interface of the two phases with a transfer pipette. Very carefully transfer this suspension into a 1. Centrifuge at 4. 00 x g for 1. C. Resuspend the samples in 1 ml of the medium. Count the number of KMNC using trypan blue exclusion on a hemocytometer and express the results as numbers of KMNC per ml per Kidney. Wash once as above. Isolation of Hematopoietic (CD4. Cells from KMNC (Step I)Resuspend the cells up to 1. Add 1. 0 µl of CD4. C. Add 1 ml of the running buffer to stop the reaction. Centrifuge at 4. 00 x g for 1. C and resuspend in 5. Prepare the magnetic column by placing at the magnet and rinse with 3 ml of running buffer. Pass the cell suspension through the magnetic column and collect the unlabeled cells that pass through the column. Wash the cells three times with 3 ml of running buffer. Then collect the effluent. Remove the column from the separator and pipette 5 ml of running buffer onto the columns and flush out the labeled fraction immediately. This fraction contains the CD4. Count the cells. To calculate the absolute number of different T cells subsets into kidneys multiply the total number of KMNC by the percentage of positive cells determined by flow cytometry (Figure 2). Isolation of DN T Cell from CD4. Preparation by Negative Selection (Step II)Add 2. CD4, anti- CD8, anti- Fc receptor (CD1. MHC class II (I- A b), anti- CD1d PBS- 5. Incubate cells for 3. Add 1 ml anti- Biotin microbeads. Incubate for 3. 0 min in a cold room. Place the tube in the magnet and proceed to depletion. Count the viable cells to determine the total number of DN T cells in the negative fraction by using a hemocytometer. Check purity of DN T cells with flow cytometry by following this gating strategy: first gate CD4. TCRαβ+. Exclude CD1d+ cells. Plot CD4+ vs. CD8+ to identify DN T cells (Figure 3). Multiply the total number by the percentage determined in purity. Note: Using this protocol results in a population of more than 9. DN T cells. Representative Results. Things Black People Confuse as Racist. America is such a global heavyweight that we forget the nation is only 2. More than a third of U. S. history is marked by the legal institution of slavery and we’ve been dealing with the fallout of racial inequality ever since it was abolished. But cultural traditions run deep and propagate down generations. While progress is steady, America’s color lines don’t erase easy. And few things warp a child’s mind more than the ridiculous notion that people don’t like you or judge you because you’re black. Even as an adult, it’s infuriating, depressing and demeaning all at once. Socially, it causes us to defend and define our existence out of habit. I’m not a “man in America” but rather a “black man in America,” and the difference is anything but subtle. Today’s racism is often subtle, unlike the strain that infected the nation during the civil rights era. ![]() It’s carefully veiled. Daily situations are more shades of grey than simply black or white. Like any form of oppression, the people on the receiving end are left with the impotence to say something. But sometimes, our learned defensiveness jumps the gun and what appears to be classic racism may actually be a case of mistaken identity. Here are the 7 most common things that get billed racist when the check should be going somewhere else. Being Confused as a Store Clerk. Ever go shopping in [insert chain store here] and a white person asks you for help? The first thing you think is, “Oh, you think I work here because I’m black?” but not so fast. Includes downloads, cheats, reviews, and articles. Travel Slideshow in February 2018 07-24-17. For many years, Henry Rollins has used his camera to document his travels around the world. Join Henry for an intimate. There are plenty of non- racist reasons someone might think you work there like your outfit. I’ve been caught out there wearing matching colors to the store clerks. Sometimes people just aren’t being mindful. Say you knock over a few boxes of cereal and start resetting them as someone walks over with a burning question, hardly looking your direction in her thirst to consume. Silly things like that happen all the time. But if you’re wearing a boardroom suit in the supermarket and some white person comes up talking about, “where’s the oatmeal,” that’s some racist s#!@“You people”I know the phrase “you people” to be quite useful to describe a group of folks that share one or more bothersome qualities, like “you people taking up all the space by the subway car door” or “you people who don’t wait till passengers get off the car before boarding” and even “you people who keep sitting on my car.” Annoying people come from all ethnic backgrounds. · Computer game Age of Empires 2 Age of Empires 2: Age of Kings Age of Empires 2: The Conquerors Computer game is A.K.A. AoE2, AoK, and Ao2: AoK. Windows 7. Download Full Version PC Games, Software's. Spread the Word.Invite your friends! This Site is Powered by TCW Communities HomePage. While it never looks good when a white person references a group of black people as “you people” it isn’t necessarily a racist statement. What’s wrong with you people? Followed in a Store. It’s easy to get racist vibes when you’re followed around a convenience store but it’s a tricky proposition. Most people, black, white, whatever, about to commit a crime are dressed for the part. A black man wearing stereotypical clothing made famous by the media (a hoodie) is more likely to be hawked in the store than one wearing a business suit. As someone that has tested this theory out on multiple occasions I can tell you its exceedingly easy to shoplift when you’re dressed for a corporate office. So when you’re getting a watchful eye from a store clerk or owner, take solace in the fact that though you are being profiled, it isn’t necessarily race related. Face paint. The practice of blackface enjoyed a long and popular run in American entertainment and didn’t become taboo until late in the 2. Now, those ignorant enough to still don shoe polish are publicly crucified or worse. But while you’ll probably always double- take when you see a white person in dark face paint it’s not necessarily racist or blackface. The last time this came on a national scale was Robert Downey Jr’s satirical role in Tropic Thunder, in which he played an white Australian playing a black American general. Describing Someone as Black. You’re talking with Bob from accounting and he’s trying to get you to remember someone from the holiday party, “you remember Jerry, the tall black guy.” And now you’re thinking, “I knew Bob was racist.” But simply mentioning someone is black isn’t the hallmark of a racist. Sometimes, it’s an effective way to remember someone. Consider the opposite, which often happens in a mixed- race conversation. A white person, who wants to remind you of a black person without mentioning that key detail for fear of being considered ignorant. She’ll use a load of adjectives that are ultimately unhelpful. You may even be left with the burning question, “is she white or black?” but leave it out to avoid going “racial.”“Everyone Looks Alike”I was in the elevator and recognized someone new in the building. I asked, “Do you remember me?” when I could tell she obviously didn’t. Blushing and embarrassed, she admitted, “I’m confused, everyone looks alike.” I might have easily taken what she said as racist (she is east Indian) but I could tell that it wasn’t–not in this case. Perhaps poorly worded, she was just remarking on the universally confusing task of getting to know new names and faces. Not Having Black Friends. Birds of a feather flock together and by and large, so do folks from similar ethnic backgrounds and skin colors. So at the end of the day, should you be surprised that the average white (or other) person doesn’t have not a single black friend? Sure, it may be a little weird, but having no black friends doesn’t make said white person any more racist than does not owning a pet make someone prone to animal cruelty though the door is always open for that possibility, in both cases. But be on the lookout. The moment you discover an acquaintance doesn’t have any black friends may mean you’re being recruited as the “token.”More on Madame Noire! ![]() Browse our quotes collection, once you found your desired one, create beautiful quote as image and share on Facebook, Twitter & Pinterest. The sheep (Ovis aries) is a quadrupedal, ruminant mammal typically kept as livestock. Like all ruminants, sheep are members of the order Artiodactyla, the even-toed. Can cognitive technologies do government employees’ thinking for them? Not quite—at least not yet. But right now, AI-based programs can help agencies cut costs. Department of Human Services - Policies, Guidelines & Legislation. Aboriginal Cultural Competence Matrix; Aboriginal Family Violence Consultants’ Guidelines. Latest Topics | ZDNet. Ericsson, Ciena, and Telstra have encrypted data while in transit over a 1. Gbps link between the US and Australia using multiple subsea cable systems, following their successful trial over a 1. Gbps link earlier this year. Corinne Reichert in Mobility. |
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